彩票app下载官网-正规彩票投注app-彩票app官方

联系我们
点击收缩

在线客服

咨询热线

027-87561033 15071267082

武汉纽斯特生特技术有限公司欢迎您!

手机店铺

neweastbio@126.com

logo

 | 027-87561033

中文

产品中心

PRODUCT CENTER

Anti Active Rac Mouse Monoclonal Antibody

价格4800.00
品牌NewEastBio   
产地美国
货号26903
免疫原Mouse
规格30μl
技术资料查看技术资料
  • 数据表
  • 参考文献
扫一扫购买
  • 概述


    Active Rac1-GTP [26903]                                                                                                                                              ¥:4800.00

    [30 µL]

    Catalog Number:  26903
    Gene Symbol:  RAC1
    Description:   Anti Active Rac1-GTP Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
    Immunogen:   Recombinant full length protein of active Rac1
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                       1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgM
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Rac1 antibody recognizes active Rac1 from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  
    Rac activation assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti-active Rac monoclonal antibody (Cat # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti-Rac rabbit polyclonal antibody (Cat # 21003). The bottom panel shows the Western blot with anti-Rac of the cell lysates used (5% of that used in the top panel).
    Immunofluorescence:  
    Immunofluorescence for the active Rac using Anti-Active Rac1-GTP Mouse Monoclonal Antibody [26903] shows Rac-GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. 
    The tissue sections were fixed with -20 ºC methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rabbit AlexaFluor594 at 1:1000.

    Related Products

    Catalog#NameSizePrice
    Rac Activation Assay Kit 20 assays ¥6800.00
    Active Rac1-GTP Monoclonal Antibody 30 μL  ¥4800.00
    Anti-Rac1 Mouse Monoclonal Antibody 100 μL  ¥2800.00
    Anti-Rac1 Rabbit Polyclonal Antibody 100 μL  ¥2600.00
    Anti-Rac2 Rabbit Polyclonal Antibody 100 μL  ¥2600.00



    Publications:
    1.  
    Cytoskeleton Volume 71, Issue 4, pages 257–272, April 2014
    2.  
    Neutrophil Methods and Protocols Methods in Molecular Biology Volume 1124, 2014, pp 251-268
    3.  
    Molecular and Cellular Neuroscience Volume 60, May 2014, Pages 10–25
    4.  
    The Journal of Immunology April 14, 2014 1301155
    5.  
    International Journal of Cancer Volume 135, Issue 11, pages 2493–2506, 1 December 2014
    6.  
    J Biol Chem. 2014 May 30;289(22):15244-58
    7.  
    Mol Cell Biol. 2014 Nov 15;34(22):4186-99
    8.  
    PLoS One. 2014 Sep 23;9(9):e108257
    9.  
    Cancer Lett. 2015 Jan 28;356(2 Pt B):382-91
    10.  
    Physiological ReportsPublished 24 October 2014Vol. 2no. e12175DOI: 10.14814/phy2.12175
    11.  
    Anticancer Research December 2014 vol. 34 no. 12 7111-7117
    12.  
    Cancer letters. March 1, 2015Volume 358, Issue 1, Pages 76–84.
    13.  
    Integr Biol (Camb). 2014 Dec 24
    14.  
    Mol Neurobiol. 2015 Jan 24


Copyright©2019武汉纽斯特生特技术有限公司版权所有      公安备案号42018502003460

技术支持:优狐