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Anti Active RhoA Mouse Monoclonal Antibody

价格4800
品牌NewEastBio   
产地美国
货号26904
免疫原Mouse
规格30μl
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  • 概述

    Active RhoA -GTP [26904]
    [30 µL]


    Catalog Number:  26904
    Gene Symbol:  RHOA
    Description:   Anti Active RhoA Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.
    Immunogen:   Recombinant full length protein of active RhoA
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                       1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG2b
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active RhoA antibody recognizes active RhoA from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western Blot:  
    IP/WB analysis of active RhoA protein. Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-active RhoA mouse monoclonal antibody (Cat. # 26904). After SDS/PAGE, the membrane filter was probed with anti-RhoA mouse monoclonal antibody (Cat. # 26007).


    Publications:
    1.  
        J Neurochem. 2011 Dec;119(6):1306-16
    2.  
        Oncogene. 2012 Aug 30;31(35):3913-23
    3.  
        Carcinogenesis. 2010 Jul;31(7):1230-7
    4.  
        Development, Growth & Differentiation. Volume 55, Issue 4, pages 579–590, May 2013
    5.  
        Cell Death and Differentiation (2013) 20, 1031–1042
    6.  
        J Biol Chem. 2013 Jul 12;288(28):20217-27
    7.  
        Diabetes. 2013 Nov;62(11):3817-27
    8.  
        Journal of Neurochemistry Volume 127, Issue 2, pages 152–162, October 2013
    9.  
        Diabetes. 2014 Feb;63(2):701-14
    10.  
        Cell Death and Disease (2013) 4, e848
    11.  
        Biochimica et Biophysica Acta (BBA) - General Subjects Volume 1840, Issue 8, August 2014, Pages 2482–2490
    12.  
        J Cell Sci. 2014 Mar 15;127(Pt 6):1229-41
    13.  
        Cytokine Volume 69, Issue 1, September 2014, Pages 47–55
    14.  
        PLoS One. 2014 Oct 16;9(10):e110851


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