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Anti Active Cdc42 Mouse Monoclonal Antibody

价格4800
品牌NewEastBio   
产地美国
货号26905
免疫原Mouse
规格30μl
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  • 概述

    Active Cdc42 GTP [26905]
    [30 µL]

    Catalog Number:  26905
    Gene Symbol:  CDC42
    Description:   Anti Active Cdc42 Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
    Immunogen:   Recombinant full length protein of active Cdc42
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                       1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG1
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti active Cdc42 antibody recognizes active Cdc42 from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    IP-Western blot:  
    IP/WB analysis of active Cdc42 protein. Purified recombinant Cdc42 proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti active Cdc42 mouse monoclonal antibody (Cat. # 26905). After SDS/PAGE, the membrane filter was probed with anti Cdc42 mouse monoclonal antibody (Cat. # 26008).
    Immunofluorescence:
    Immunofluorescent labeling of Cdc42-GTP in MS1 mouse endothelial cells. The MS1 cells cultured on glass coverslips (Fisher) in DMEM containing 5% FBS were fixed with 4% paraformaldehyde for 10 minutes at RT (room temperature) and permeablized in PBSN (PBS+0.1%NP40) for 15 minutes at RT. The cells were stained with primary antibody (Cdc42 GTP) at 1:25 dilution in Invitrogen's CAS-BLOCK (00-8120) reagent at RT for 1 hour. After brief washing in PBSN (3x5min), a secondary Alexa555 conjugated goat anti mouse antibody (Invitrogen A21424) was applied in 1:200 in CAS-BLOCK at RT for 1 hour. The coverslips were then washed in PBSN (3x5min) and mounted using vectorsheld's hard set mounting medium (H-1500), and examined using a Zeiss inverted epifluorescent miscroscope.


    Related Products
    Catalog#NameSizePrice
    80701Cdc42 Activation Assay Kit 20 assays¥6,800
    26905Active Cdc42 GTP Monoclonal Antibody 30 μL¥4,800
    26008Anti Cdc42 Mouse Monoclonal Antibody 100 μL ¥2800  
    21010Anti Cdc42 Rabbit Polyclonal Antibody 100 μL ¥2600  


    Publications:
    1.  
        PLoS Pathog. 2013 Jan;9(1):e1003142
    2.  
        Cytokine. 2012 Jan;57(1):158-68
    3.  
        European Journal of Cell Biology Volume 91, Issues 11–12, November–December 2012, Pages 978–987
    4.  
        J Cell Biol. 2012 Aug 20;198(4):677-93
    5.  
        Am J Physiol Lung Cell Mol Physiol. 2011 Jan;300(1):L32-42
    6.  
        Mol Cell Biol. 2011 Nov;31(22):4430-41
    7.  
        PLoS One. 2013 Jul 19;8(7):e70123
    8.  
        PLoS One. 2013 Sep 3;8(9):e73063
    9.  
        J Biol Chem. 2014 Jun 27;289(26):18347-59
    10.  
        United States Patent Application 20140194451
    11.  
        Molecular Pharmacology January 2014 vol. 85 no. 1 50-61


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