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Anti Active RhoA Mouse Monoclonal Antibody

价格4800.00
品牌NewEastBio   
产地美国
货号26904
免疫原Mouse
规格30μl
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  • 概述

    Active RhoA GTP [26904]
    [30 µL]


    Catalog Number:  26904
    Gene Symbol:  RHOA
    Description:   Anti Active RhoA Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.
    Immunogen:   Recombinant full length protein of active RhoA
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                       1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG2b
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active RhoA antibody recognizes active RhoA from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western Blot:  
    IP/WB analysis of active RhoA protein. Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti active RhoA mouse monoclonal antibody (Cat. # 26904). After SDS/PAGE, the membrane filter was probed with anti RhoA mouse monoclonal antibody (Cat. # 26007).


    Related Products
    Catalog#NameSizePrice
    80601RhoA Activation Assay Kit 20 assays ¥6800  
    26904Active RhoA GTP Monoclonal Antibody 30 μL ¥4800
    26007Anti RhoA Mouse Monoclonal Antibody 100 μL ¥2800
    21009Anti RhoA Rabbit Polyclonal Antibody 100 μL ¥2600


    Publications:
    1.  
        J Neurochem. 2011 Dec;119(6):1306-16
    2.  
        Oncogene. 2012 Aug 30;31(35):3913-23
    3.  
        Carcinogenesis. 2010 Jul;31(7):1230-7
    4.  
        Development, Growth & Differentiation. Volume 55, Issue 4, pages 579–590, May 2013
    5.  
        Cell Death and Differentiation (2013) 20, 1031–1042
    6.  
        J Biol Chem. 2013 Jul 12;288(28):20217-27
    7.  
        Diabetes. 2013 Nov;62(11):3817-27
    8.  
        Journal of Neurochemistry Volume 127, Issue 2, pages 152–162, October 2013
    9.  
        Diabetes. 2014 Feb;63(2):701-14
    10.  
        Cell Death and Disease (2013) 4, e848
    11.  
        Biochimica et Biophysica Acta (BBA) - General Subjects Volume 1840, Issue 8, August 2014, Pages 2482–2490
    12.  
        J Cell Sci. 2014 Mar 15;127(Pt 6):1229-41
    13.  
        Cytokine Volume 69, Issue 1, September 2014, Pages 47–55
    14.  
        PLoS One. 2014 Oct 16;9(10):e110851


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